Usitatibacter rugosus gen. nov., sp. nov. and Usitatibacter palustris sp. nov., novel members of Usitatibacteraceae fam. nov. within the order Nitrosomonadales isolated from soil

@article{Vieira2021,
  Title = {Usitatibacter rugosus gen. nov., sp. nov. and Usitatibacter palustris sp. nov., novel members of Usitatibacteraceae fam. nov. within the order Nitrosomonadales isolated from soil},
  Author = {Vieira, Selma and Huber, Katharina J and Neumann-Schaal, Meina and Geppert, Alicia and Luckner, Manja and Wanner, Gerhard and Overmann, Jorg},
  Editor = {},
  Journal = {International journal of systematic and evolutionary microbiology},
  Year = {2021},
  Volume = {71},
  Doi = {10.1099/ijsem.0.004631},
  Abstract = {Members of the metabolically diverse order Nitrosomonadales inhabit a wide range of environments. Two strains affiliated with this order were isolated from soils in Germany and characterized by a polyphasic approach. Cells of strains 0125_3 T and Swamp67 T are Gram-negative rods, non-motile, non-spore-forming, non-capsulated and divide by binary fission. They tested catalase-negative, but positive for cytochrome c -oxidase. Both strains form small white colonies on agar plates and grow aerobically and chemoorganotrophically on SSE/HD 1 : 10 medium, preferably utilizing organic acids and proteinaceous substrates. Strains 0125_3 T and Swamp67 T are mesophilic and grow optimally without NaCl addition at slightly alkaline conditions. Major fatty acids are C 16 : 1 ω 7 c , C 16 : 0 and C 14 : 0 . The major polar lipids are diphosphatidylglycerol, phosphatidylethanolamine and phosphatidyglycerol. The predominant respiratory quinone is Q-8. The G+C content for 0125_3 T and Swamp67 T was 67 and 66.1 %, respectively. The 16S rRNA gene analysis indicated that the closest relatives (<91 % sequence similarity) of strain 0125_3 T were Nitrosospira multiformis ATCC 25196 T , Methyloversatilis universalis FAM5 T and Denitratisoma oestradiolicum AcBE2-1 T , while Nitrosospira multiformis ATCC 25196 T , Nitrosospira tenuis Nv1 T and Nitrosospira lacus APG3 T were closest to strain Swamp67 T . The two novel strains shared 97.4 % 16S rRNA gene sequence similarity with one another and show low average nucleotide identity of their genomes (83.8 %). Based on the phenotypic, chemotaxonomic, genomic and phylogenetic analysis, we propose the two novel species Usitatibacter rugosus sp. nov (type strain 0125_3 T =DSM 104443 T =LMG 29998 T =CECT 9241 T ) and Usitatibacter palustris sp. nov. (type strain Swamp67 T =DSM 104440 T =LMG 29997 T =CECT 9242 T ) of the novel genus Usitatibacter gen. nov., within the novel family Usitatibacteraceae fam. nov.},
}

TY  - JOUR
AU  - Vieira, Selma
AU  - Huber, Katharina J
AU  - Neumann-Schaal, Meina
AU  - Geppert, Alicia
AU  - Luckner, Manja
AU  - Wanner, Gerhard
AU  - Overmann, Jorg
TI  - Usitatibacter rugosus gen. nov., sp. nov. and Usitatibacter palustris sp. nov., novel members of Usitatibacteraceae fam. nov. within the order Nitrosomonadales isolated from soil
T2  - International journal of systematic and evolutionary microbiology
PY  - 2021
VL  - 71
DO  - 10.1099/ijsem.0.004631
AB  - Members of the metabolically diverse order Nitrosomonadales inhabit a wide range of environments. Two strains affiliated with this order were isolated from soils in Germany and characterized by a polyphasic approach. Cells of strains 0125_3 T and Swamp67 T are Gram-negative rods, non-motile, non-spore-forming, non-capsulated and divide by binary fission. They tested catalase-negative, but positive for cytochrome c -oxidase. Both strains form small white colonies on agar plates and grow aerobically and chemoorganotrophically on SSE/HD 1 : 10 medium, preferably utilizing organic acids and proteinaceous substrates. Strains 0125_3 T and Swamp67 T are mesophilic and grow optimally without NaCl addition at slightly alkaline conditions. Major fatty acids are C 16 : 1 ω 7 c , C 16 : 0 and C 14 : 0 . The major polar lipids are diphosphatidylglycerol, phosphatidylethanolamine and phosphatidyglycerol. The predominant respiratory quinone is Q-8. The G+C content for 0125_3 T and Swamp67 T was 67 and 66.1 %, respectively. The 16S rRNA gene analysis indicated that the closest relatives (<91 % sequence similarity) of strain 0125_3 T were Nitrosospira multiformis ATCC 25196 T , Methyloversatilis universalis FAM5 T and Denitratisoma oestradiolicum AcBE2-1 T , while Nitrosospira multiformis ATCC 25196 T , Nitrosospira tenuis Nv1 T and Nitrosospira lacus APG3 T were closest to strain Swamp67 T . The two novel strains shared 97.4 % 16S rRNA gene sequence similarity with one another and show low average nucleotide identity of their genomes (83.8 %). Based on the phenotypic, chemotaxonomic, genomic and phylogenetic analysis, we propose the two novel species Usitatibacter rugosus sp. nov (type strain 0125_3 T =DSM 104443 T =LMG 29998 T =CECT 9241 T ) and Usitatibacter palustris sp. nov. (type strain Swamp67 T =DSM 104440 T =LMG 29997 T =CECT 9242 T ) of the novel genus Usitatibacter gen. nov., within the novel family Usitatibacteraceae fam. nov.
ER  -