Amplification‐ and Enzyme‐Free Magnetic Diagnostics Circuit for Whole‐Genome Detection of SARS‐CoV‐2 RNA

• Enja Laureen Rösch
• Rebecca Sack
• Mohammad Suman Chowdhury
• Florian Wolgast
• Margarete Zaborski
• Frank Ludwig
• Meinhard Schilling
• Thilo Viereck
• Ulfert Rand
• Aidin Lak

Departments

Human and Animal Cell Lines

Abstract

Polymerase chain reaction (PCR) requires thermal cycling and enzymatic reactions for sequence amplification, hampering their applications in point‐of‐care (POC) settings. Magnetic bioassays based on magnetic particle spectroscopy (MPS) and magnetic nanoparticles (MNPs) are isothermal, wash‐free, and can be quantitative. Realizing them amplification‐ and enzyme‐free on a benchtop device, they will become irreplaceable for POC applications. Here we demonstrate a first‐in‐class magnetic signal amplification circuit (MAC) that enables detection of whole genome of SARS‐CoV‐2 by combining the specificity of toehold‐mediated DNA strand displacement with the magnetic response of MNPs to declustering processes. Using MAC, we detect the N gene of SARS‐CoV‐2 samples at a concentration of 104 RNA copies/µl as determined by droplet digital PCR. Further, we demonstrate that MAC can reliably distinguish between SARS‐CoV‐2 and other human coronaviruses. Being a wash‐, amplification‐ and enzyme‐free biosensing concept and working at isothermal conditions (25 °C) on a low‐cost benchtop MPS device, our MAC biosensing concept offers several indispensable features for translating nucleic acid detection to POC applications.

• DOI: 10.1002/cbic.202400251

Cite this activity

@article{Rösch2024,
  Title = {Amplification‐ and Enzyme‐Free Magnetic Diagnostics Circuit for Whole‐Genome Detection of SARS‐CoV‐2 RNA},
  Author = {Rösch, Enja Laureen and Sack, Rebecca and Chowdhury, Mohammad Suman and Wolgast, Florian and Zaborski, Margarete and Ludwig, Frank and Schilling, Meinhard and Viereck, Thilo and Rand, Ulfert and Lak, Aidin},
  Editor = {},
  Journal = {Chembiochem : a European journal of chemical biology},
  Year = {2024},
  Doi = {10.1002/cbic.202400251},
  Abstract = {Polymerase chain reaction (PCR) requires thermal cycling and enzymatic reactions for sequence amplification, hampering their applications in point‐of‐care (POC) settings. Magnetic bioassays based on magnetic particle spectroscopy (MPS) and magnetic nanoparticles (MNPs) are isothermal, wash‐free, and can be quantitative. Realizing them amplification‐ and enzyme‐free on a benchtop device, they will become irreplaceable for POC applications. Here we demonstrate a first‐in‐class magnetic signal amplification circuit (MAC) that enables detection of whole genome of SARS‐CoV‐2 by combining the specificity of toehold‐mediated DNA strand displacement with the magnetic response of MNPs to declustering processes. Using MAC, we detect the N gene of SARS‐CoV‐2 samples at a concentration of 104 RNA copies/µl as determined by droplet digital PCR. Further, we demonstrate that MAC can reliably distinguish between SARS‐CoV‐2 and other human coronaviruses. Being a wash‐, amplification‐ and enzyme‐free biosensing concept and working at isothermal conditions (25 °C) on a low‐cost benchtop MPS device, our MAC biosensing concept offers several indispensable features for translating nucleic acid detection to POC applications.},
}

TY  - JOUR
AU  - Rösch, Enja Laureen
AU  - Sack, Rebecca
AU  - Chowdhury, Mohammad Suman
AU  - Wolgast, Florian
AU  - Zaborski, Margarete
AU  - Ludwig, Frank
AU  - Schilling, Meinhard
AU  - Viereck, Thilo
AU  - Rand, Ulfert
AU  - Lak, Aidin
TI  - Amplification‐ and Enzyme‐Free Magnetic Diagnostics Circuit for Whole‐Genome Detection of SARS‐CoV‐2 RNA
T2  - Chembiochem : a European journal of chemical biology
PY  - 2024
DO  - 10.1002/cbic.202400251
AB  - Polymerase chain reaction (PCR) requires thermal cycling and enzymatic reactions for sequence amplification, hampering their applications in point‐of‐care (POC) settings. Magnetic bioassays based on magnetic particle spectroscopy (MPS) and magnetic nanoparticles (MNPs) are isothermal, wash‐free, and can be quantitative. Realizing them amplification‐ and enzyme‐free on a benchtop device, they will become irreplaceable for POC applications. Here we demonstrate a first‐in‐class magnetic signal amplification circuit (MAC) that enables detection of whole genome of SARS‐CoV‐2 by combining the specificity of toehold‐mediated DNA strand displacement with the magnetic response of MNPs to declustering processes. Using MAC, we detect the N gene of SARS‐CoV‐2 samples at a concentration of 104 RNA copies/µl as determined by droplet digital PCR. Further, we demonstrate that MAC can reliably distinguish between SARS‐CoV‐2 and other human coronaviruses. Being a wash‐, amplification‐ and enzyme‐free biosensing concept and working at isothermal conditions (25 °C) on a low‐cost benchtop MPS device, our MAC biosensing concept offers several indispensable features for translating nucleic acid detection to POC applications.
ER  - 

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