Unravelling the effects of fungicides on the composition of the leaf litter associated aquatic mycobiome
Steffen Carl1, Christiane Baschien2, René Sahm1,3, and Silvia Mohr1
1German Environment Agency, Section IV 2.5 Trace Analysis, Artificial Streams and Ponds, Schichauweg 58, 12307 Berlin, Germany
bLeibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures GmbH, Department Bioresources for Bioeconomy and Health Research, Inhoffenstraße 7B, 38124 Braunschweig, Germany
cUniversity of Kassel, Department Freshwater Ecology in Landscape Planning, Gottschalkstraße 26a, 34127 Kassel, Germany.
Aquatic fungi play a key role in the turnover of organic matter in freshwater ecosystems, such as leaf litter in streams. Fungicides that reach streams via spray drift or surface runoff from agricultural activities may endanger the diversity of these organisms. Therefore, we assess composition changes of the aquatic fungal community on Alnus glutinosa leaf litter in micro- and mesocosms treated with model fungicides in comparison to untreated controls and field conditions. For this purpose, we use high-throughput sequencing (HTS) accompanied with sporulation and isolation of pure cultures in order to identify possible indicator organisms and study their robustness towards fungicide stress. Our results confirm, that certain aquatic fungal species react with different sensitivities to fungicide exposure. With regard to the suitability of HTS for the analysis of community changes, the results suggest that addressing eDNA is particularly appropriate to detect fungicide effects during the colonization process of leaf litter, a crucial process for the following decomposition and the conservation of fungal diversity. On the other hand, conditioning of leaf litter under artificial conditions even in mesocosms led to less complex communities compared to a reference stream, still limiting the transferability to natural conditions. Future research could address e. g. further omics strategies to corroborate our findings.
Carl S., Baschien C., Sahms R. and Mohr S. Unravelling the effects of fungicides on the composition of the leaf litter associated aquatic mycobiome. IMC12, IMC12 Maastricht, Niederlande. 12.-15.08.2024. (Invited lecture: long)
@misc{Carl2024,
Title = {Unravelling the effects of fungicides on the composition of the leaf litter associated aquatic mycobiome},
Author = {Carl, Steffen and Baschien, Christiane and Sahms, René and Mohr, Silvia},
Editor = {},
Year = {2024},
Abstract = {Unravelling the effects of fungicides on the composition of the leaf litter associated aquatic mycobiome
Steffen Carl1, Christiane Baschien2, René Sahm1,3, and Silvia Mohr1
1German Environment Agency, Section IV 2.5 Trace Analysis, Artificial Streams and Ponds, Schichauweg 58, 12307 Berlin, Germany
bLeibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures GmbH, Department Bioresources for Bioeconomy and Health Research, Inhoffenstraße 7B, 38124 Braunschweig, Germany
cUniversity of Kassel, Department Freshwater Ecology in Landscape Planning, Gottschalkstraße 26a, 34127 Kassel, Germany.
Aquatic fungi play a key role in the turnover of organic matter in freshwater ecosystems, such as leaf litter in streams. Fungicides that reach streams via spray drift or surface runoff from agricultural activities may endanger the diversity of these organisms. Therefore, we assess composition changes of the aquatic fungal community on Alnus glutinosa leaf litter in micro- and mesocosms treated with model fungicides in comparison to untreated controls and field conditions. For this purpose, we use high-throughput sequencing (HTS) accompanied with sporulation and isolation of pure cultures in order to identify possible indicator organisms and study their robustness towards fungicide stress. Our results confirm, that certain aquatic fungal species react with different sensitivities to fungicide exposure. With regard to the suitability of HTS for the analysis of community changes, the results suggest that addressing eDNA is particularly appropriate to detect fungicide effects during the colonization process of leaf litter, a crucial process for the following decomposition and the conservation of fungal diversity. On the other hand, conditioning of leaf litter under artificial conditions even in mesocosms led to less complex communities compared to a reference stream, still limiting the transferability to natural conditions. Future research could address e. g. further omics strategies to corroborate our findings.},
}
TY - SLIDE
AU - Carl, Steffen
AU - Baschien, Christiane
AU - Sahms, René
AU - Mohr, Silvia
TI - Unravelling the effects of fungicides on the composition of the leaf litter associated aquatic mycobiome
PY - 2024
AB - Unravelling the effects of fungicides on the composition of the leaf litter associated aquatic mycobiome
Steffen Carl1, Christiane Baschien2, René Sahm1,3, and Silvia Mohr1
1German Environment Agency, Section IV 2.5 Trace Analysis, Artificial Streams and Ponds, Schichauweg 58, 12307 Berlin, Germany
bLeibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures GmbH, Department Bioresources for Bioeconomy and Health Research, Inhoffenstraße 7B, 38124 Braunschweig, Germany
cUniversity of Kassel, Department Freshwater Ecology in Landscape Planning, Gottschalkstraße 26a, 34127 Kassel, Germany.
Aquatic fungi play a key role in the turnover of organic matter in freshwater ecosystems, such as leaf litter in streams. Fungicides that reach streams via spray drift or surface runoff from agricultural activities may endanger the diversity of these organisms. Therefore, we assess composition changes of the aquatic fungal community on Alnus glutinosa leaf litter in micro- and mesocosms treated with model fungicides in comparison to untreated controls and field conditions. For this purpose, we use high-throughput sequencing (HTS) accompanied with sporulation and isolation of pure cultures in order to identify possible indicator organisms and study their robustness towards fungicide stress. Our results confirm, that certain aquatic fungal species react with different sensitivities to fungicide exposure. With regard to the suitability of HTS for the analysis of community changes, the results suggest that addressing eDNA is particularly appropriate to detect fungicide effects during the colonization process of leaf litter, a crucial process for the following decomposition and the conservation of fungal diversity. On the other hand, conditioning of leaf litter under artificial conditions even in mesocosms led to less complex communities compared to a reference stream, still limiting the transferability to natural conditions. Future research could address e. g. further omics strategies to corroborate our findings.
CY - IMC12 Maastricht, Niederlande
ER -
