@article{Weinberger2025,
Title = {Expanding the cultivable human archaeome: Methanobrevibacter intestini sp. nov. and strain Methanobrevibacter smithii ‘GRAZ-2’ from human faeces},
Author = {Weinberger, Viktoria and Mohammadzadeh, Rokhsareh and Blohs, Marcus and Kalt, Kerstin and Mahnert, Alexander and Moser, Sarah and Cecovini, Marina and Mertelj, Polona and Zurabishvili, Tamara and Arora, Bhawna and Wolf, Jacqueline and Shinde, Tejus and Madl, Tobias and Habisch, Hansjörg and Kolb, Dagmar and Pernitsch, Dominique and Hingerl, Kerstin and Metcalf, William and Moissl-Eichinger, Christine},
Editor = {},
Journal = {International journal of systematic and evolutionary microbiology},
Year = {2025},
Volume = {75},
Doi = {10.1099/ijsem.0.006751},
Abstract = {Two mesophilic, hydrogenotrophic methanogens, WWM1085 and M. smithii GRAZ-2, were isolated from human faecal samples. WWM1085 was isolated from an individual in the United States and represents a novel species within the genus Methanobrevibacter. M. smithii GRAZ-2 (=DSM 116045) was retrieved from a faecal sample of a European, healthy woman and represents a novel strain within this species. Both Methanobrevibacter representatives form non-flagellated, short rods with variable morphologies and the capacity to form filaments. Both isolates showed the typical fluorescence of F420 and methane production. Compared to M. smithii GRAZ-2, WWM1085 did not accumulate formate when grown with H2 and CO2. The optimal growth conditions were at 35–39 °C and pH 6.5–7.5. Full genome sequencing revealed a genomic difference of WWM1085 to the type strain of M. smithii DSM 861 (=PST), with 93.55% average nucleotide identity (ANI) and major differences in the sequence of its mcrA gene (3.3% difference in nucleotide sequence). Differences in the 16S rRNA gene sequence were very minor, and thus distinction based on this gene marker might not be possible. M. smithii GRAZ-2 was identified as a novel strain within the species Methanobrevibacter smithii (ANI 99.04% to M. smithii DSM 861 [=PST]). Due to the major differences between WWM1085 and M. smithii type strain M. smithii DSM 861 (=PST) in phenotypic, genomic and metabolic features, we propose Methanobrevibacter intestini sp. nov. as a novel species with WWM1085 as the type strain (DSM 116060T = CECT 30992T).},
}
TY - JOUR
AU - Weinberger, Viktoria
AU - Mohammadzadeh, Rokhsareh
AU - Blohs, Marcus
AU - Kalt, Kerstin
AU - Mahnert, Alexander
AU - Moser, Sarah
AU - Cecovini, Marina
AU - Mertelj, Polona
AU - Zurabishvili, Tamara
AU - Arora, Bhawna
AU - Wolf, Jacqueline
AU - Shinde, Tejus
AU - Madl, Tobias
AU - Habisch, Hansjörg
AU - Kolb, Dagmar
AU - Pernitsch, Dominique
AU - Hingerl, Kerstin
AU - Metcalf, William
AU - Moissl-Eichinger, Christine
TI - Expanding the cultivable human archaeome: Methanobrevibacter intestini sp. nov. and strain Methanobrevibacter smithii ‘GRAZ-2’ from human faeces
T2 - International journal of systematic and evolutionary microbiology
PY - 2025
VL - 75
DO - 10.1099/ijsem.0.006751
AB - Two mesophilic, hydrogenotrophic methanogens, WWM1085 and M. smithii GRAZ-2, were isolated from human faecal samples. WWM1085 was isolated from an individual in the United States and represents a novel species within the genus Methanobrevibacter. M. smithii GRAZ-2 (=DSM 116045) was retrieved from a faecal sample of a European, healthy woman and represents a novel strain within this species. Both Methanobrevibacter representatives form non-flagellated, short rods with variable morphologies and the capacity to form filaments. Both isolates showed the typical fluorescence of F420 and methane production. Compared to M. smithii GRAZ-2, WWM1085 did not accumulate formate when grown with H2 and CO2. The optimal growth conditions were at 35–39 °C and pH 6.5–7.5. Full genome sequencing revealed a genomic difference of WWM1085 to the type strain of M. smithii DSM 861 (=PST), with 93.55% average nucleotide identity (ANI) and major differences in the sequence of its mcrA gene (3.3% difference in nucleotide sequence). Differences in the 16S rRNA gene sequence were very minor, and thus distinction based on this gene marker might not be possible. M. smithii GRAZ-2 was identified as a novel strain within the species Methanobrevibacter smithii (ANI 99.04% to M. smithii DSM 861 [=PST]). Due to the major differences between WWM1085 and M. smithii type strain M. smithii DSM 861 (=PST) in phenotypic, genomic and metabolic features, we propose Methanobrevibacter intestini sp. nov. as a novel species with WWM1085 as the type strain (DSM 116060T = CECT 30992T).
ER -
