BRENDA (BRaunschweig ENzyme DAtabase) is the world's most comprehensive online database for functional, biochemical and molecular biological data on enzymes, metabolites and metabolic pathways. It contains data on the properties, function and significance of all enzymes classified by the Enzyme Commission of the International Union of Biochemistry and Molecular Biology (IUBMB). As ELIXIR Core Data Resource and Global Core Biodata Resource, BRENDA is considered a data resource of critical importance to the international life sciences research community. The database compiles a representative overview of enzymes and metabolites using current research data from primary scientific literature and thus serves the purpose of facilitating information retrieval for researchers.

The data were extracted from more than 173,000 scientific publications that have been carefully evaluated by curators.

In total, BRENDA contains approximately 5.8 million individual data points. These refer to around 112,000 different enzymes from about 15,000 different organisms. It also includes information on approximately 278,000 different molecules that can interact with the enzymes.